APOA4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APOA4.
Immunogen
A synthetic peptide corresponding to human APOA4.
Sequence
C-KEKESQDKTLSLP
Host
Goat
Theoretical MW (kDa)
45.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
APOA4 polyclonal antibody (Cat # PAB7184) (0.01 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APOA4
Entrez GeneID
337Protein Accession#
NP_000473.2Gene Name
APOA4
Gene Alias
MGC142154, MGC142156
Gene Description
apolipoprotein A-IV
Omim ID
107690Gene Ontology
HyperlinkGene Summary
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Apolipoprotein A-IV attenuates oxidant-induced apoptosis in mitotic competent, undifferentiated cells by modulating intracellular glutathione redox balance.
Spaulding HL, Saijo F, Turnage RH, Alexander JS, Aw TY, Kalogeris TJ.
American Journal of Physiology. Cell Physiology 2006 Jan; 290(1):C95.
Application:Treated, Recombinant protein.
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Apolipoprotein A-IV attenuates oxidant-induced apoptosis in mitotic competent, undifferentiated cells by modulating intracellular glutathione redox balance.
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