GAPDH polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GAPDH polyclonal antibody (Cat # PAB6936) (0.1 ug/mL) staining of Human Liver (1), (0.03 ug/mL) Testes (2), Tonsil (3), (0.1 ug/mL) Mouse Liver (4), (0.03 ug/mL) Rat Heart (5) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
GAPDH polyclonal antibody (Cat # PAB6936) (0.03 ug/mL) staining of HeLa (1) and NIH3T3 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminesce
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GAPDH polyclonal antibody (Cat # PAB6936) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence
GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence
GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GAPDH.
Immunogen
A synthetic peptide corresponding to internal region of human GAPDH.
Sequence
C-GVNHEKYDNSLK
Host
Goat
Theoretical MW (kDa)
36.1
Reactivity
Human, Mouse, Rat
Specificity
GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunofluorescence (5-10 ug/mL)
Immunohistochemistry(2 ug/mL)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GAPDH polyclonal antibody (Cat # PAB6936) (0.1 ug/mL) staining of Human Liver (1), (0.03 ug/mL) Testes (2), Tonsil (3), (0.1 ug/mL) Mouse Liver (4), (0.03 ug/mL) Rat Heart (5) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
GAPDH polyclonal antibody (Cat # PAB6936) (0.03 ug/mL) staining of HeLa (1) and NIH3T3 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminesceImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GAPDH polyclonal antibody (Cat # PAB6936) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Immunofluorescence
GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GAPDH
Entrez GeneID
2597Protein Accession#
NP_0020.37.2Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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Mind bomb 2 limits inflammatory dermatitis in Sharpin mutant mice independently of cell death.
Daniel S Simpson, Holly Anderton, Jumana Yousef, Vineet Vaibhav, Simon A Cobbold, Esther Bandala-Sanchez, Andrew J Kueh, Laura F Dagley, Marco J Herold, John Silke, James E Vince, Rebecca Feltham.
PNAS Nexus 2023 Dec; 3(1):pgad438.
Application：WB, Mice, Mice Skin tissue.
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Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.
Ismail SA, Park HW.
Acta Crystallographica. Section D, Biological Crystallography 2005 Nov; 61(Pt 11):1508.
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Mind bomb 2 limits inflammatory dermatitis in Sharpin mutant mice independently of cell death.
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