FOXC1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
FOXC1 polyclonal antibody (Cat # PAB6425) staining (0.5 ug/mL) of human bone marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXC1 polyclonal antibody (Cat # PAB6425, 3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FOXC1.
Immunogen
A synthetic peptide corresponding to C-terminus of human FOXC1.
Sequence
RTSGAFVYDCSKF
Host
Goat
Theoretical MW (kDa)
56.8
Reactivity
Human, Mouse, Zebra fish
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (0.5-1.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Tissue lysate)
FOXC1 polyclonal antibody (Cat # PAB6425) staining (0.5 ug/mL) of human bone marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXC1 polyclonal antibody (Cat # PAB6425, 3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXC1
Entrez GeneID
2296Protein Accession#
NP_001444.2Gene Name
FOXC1
Gene Alias
ARA, FKHL7, FREAC-3, FREAC3, IGDA, IHG1, IRID1
Gene Description
forkhead box C1
Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq
Other Designations
OTTHUMP00000017802|forkhead winged/helix transcription factor|forkhead, drosophila, homolog-like 7|forkhead-related activator 3|forkhead/winged helix-like transcription factor 7|myeloid factor-delta|transcription factor forkhead-like 7
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Interactome
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Disease
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Publication Reference
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Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth.
Zhou Y, Kato H, Asanoma K, Kondo H, Arima T, Kato K, Matsuda T, Wake N.
Genomics 2002 Nov; 80(5):465.
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Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth.
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