FOXA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
FOXA1 polyclonal antibody (Cat # PAB6418) staining (1 ul/mL) of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXA1 polyclonal antibody (Cat # PAB6418) (5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FOXA1.
Immunogen
A synthetic peptide corresponding to C-terminus of human FOXA1.
Sequence
C-GVYSRPVLNTS
Host
Goat
Theoretical MW (kDa)
49.1
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.2-0.6 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
FOXA1 polyclonal antibody (Cat # PAB6418) staining (1 ul/mL) of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FOXA1 polyclonal antibody (Cat # PAB6418) (5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXA1
Entrez GeneID
3169Protein Accession#
NP_004487.2Gene Name
FOXA1
Gene Alias
HNF3A, MGC33105, TCF3A
Gene Description
forkhead box A1
Omim ID
602294Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq
Other Designations
hepatocyte nuclear factor 3, alpha
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Interactome
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Disease
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Publication Reference
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Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.
Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M.
Cell 2005 Jul; 122(1):33.
Application：WB-Tr, Human, MCF-7 cells.
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Transcription factor interactions and chromatin modifications associated with p53-mediated, developmental repression of the alpha-fetoprotein gene.
Nguyen TT, Cho K, Stratton SA, Barton MC.
Molecular and Cellular Biology 2005 Mar; 25(6):2147.
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Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.
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