SMARCA3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
SMARCA3 polyclonal antibody (Cat # PAB6172) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Flow Cytometry
SMARCA3 polyclonal antibody (Cat # PAB6172) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMARCA3.
Immunogen
A synthetic peptide corresponding to human SMARCA3.
Sequence
C-KQAKINEIRTLIDL
Host
Goat
Theoretical MW (kDa)
114
Reactivity
Human
Specificity
NP_003062.2 and NP_620636.2 are varients that represent the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Flow Cytometry (10 ug/mL)
Western blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SMARCA3 polyclonal antibody (Cat # PAB6172) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
SMARCA3 polyclonal antibody (Cat # PAB6172) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — HLTF
Entrez GeneID
6596Protein Accession#
NP_003062.2;NP_620636.1Gene Name
HLTF
Gene Alias
HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1
Gene Description
helicase-like transcription factor
Omim ID
603257Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq
Other Designations
DNA-binding protein/plasminogen activator inhibitor-1 regulator|SNF2-like 3|SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3|sucrose nonfermenting-like 3
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Interactome
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Publication Reference
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Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter.
Sheridan PL, Schorpp M, Voz ML, Jones KA.
The Journal of Biological Chemistry 1995 Mar; 270(9):4575.
Application：IP, WB-Ce, Human, HeLa cells.
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Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter.
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