CDKN1B polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CDKN1B polyclonal antibody (Cat # PAB5497) .
Lane 1 : A2780 cells.
Lane 2 : COLO 205 cells.
Lane 3 : COLO 205 cells using CDKN1B polyclonal antibody (Cat # PAB5497) preincubated with synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDKN1B polyclonal antibody (Cat # PAB5497) .
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDKN1B.
Immunogen
A synthetic peptide corresponding to residues surrounding S10 of human CDKN1B.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis using CDKN1B polyclonal antibody (Cat # PAB5497) .
Lane 1 : A2780 cells.
Lane 2 : COLO 205 cells.
Lane 3 : COLO 205 cells using CDKN1B polyclonal antibody (Cat # PAB5497) preincubated with synthesized peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDKN1B polyclonal antibody (Cat # PAB5497) . -
Gene Info — CDKN1B
Entrez GeneID
1027Gene Name
CDKN1B
Gene Alias
CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Gene Description
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
Other Designations
cyclin-dependent kinase inhibitor 1B
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis.
Connor MK, Kotchetkov R, Cariou S, Resch A, Lupetti R, Beniston RG, Melchior F, Hengst L, Slingerland JM.
Molecular Biology of the Cell 2003 Jan; 14(1):201.
Application：IP-WB, WB-Ce, Human, MCF-7 cells.
-
Novel p27(kip1) C-terminal scatter domain mediates Rac-dependent cell migration independent of cell cycle arrest functions.
McAllister SS, Becker-Hapak M, Pintucci G, Pagano M, Dowdy SF.
Molecular and Cellular Biology 2003 Jan; 23(1):216.
-
Jab1 co-activation of c-Jun is abrogated by the serine 10-phosphorylated form of p27Kip1.
Chopra S, Fernandez De Mattos S, Lam EW, Mann DJ.
The Journal of Biological Chemistry 2002 Jul; 277(36):32413.
-
CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com