YY1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of YY1 polyclonal antibody (Cat # PAB4361) in mouse kidney tissue lysate . YY1 (arrow) was detected using purified YY1 polyclonal antibody (Cat # PAB4361) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western Blot (Cell lysate)
Western blot analysis of YY1 polyclonal antibody (Cat # PAB4361) in HL-60 cell lysate . YY1 (arrow) was detected using purified YY1 polyclonal antibody (Cat # PAB4361) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with YY1 polyclonal antibody (Cat # PAB4361) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YY1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human YY1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of YY1 polyclonal antibody (Cat # PAB4361) in mouse kidney tissue lysate . YY1 (arrow) was detected using purified YY1 polyclonal antibody (Cat # PAB4361) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Western Blot (Cell lysate)
Western blot analysis of YY1 polyclonal antibody (Cat # PAB4361) in HL-60 cell lysate . YY1 (arrow) was detected using purified YY1 polyclonal antibody (Cat # PAB4361) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with YY1 polyclonal antibody (Cat # PAB4361) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — YY1
Entrez GeneID
7528Protein Accession#
P25490Gene Name
YY1
Gene Alias
DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1
Gene Description
YY1 transcription factor
Omim ID
600013Gene Ontology
HyperlinkGene Summary
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq
Other Designations
INO80 complex subunit S|Yin and Yang 1 protein
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Interactome
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Disease
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Publication Reference
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Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation.
Kurisaki K, Kurisaki A, Valcourt U, Terentiev AA, Pardali K, Ten Dijke P, Heldin CH, Ericsson J, Moustakas A.
Molecular and Cellular Biology 2003 Jul; 23(13):4494.
Application：IP, IP-WB, WB-Ce, WB-Tr, Human, Monkey, MDA-MB-468, HaCaT, COS-7 cells.
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Modulation of YY1 activity by SAP30.
Huang NE, Lin CH, Lin YS, Yu WC.
Biochemical and Biophysical Research Communications 2003 Jun; 306(1):267.
Application：IP, WB-Ce, Human, HeLa, H1299 cells.
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Yin Yang 1 is increased in human heart failure and represses the activity of the human alpha-myosin heavy chain promoter.
Sucharov CC, Mariner P, Long C, Bristow M, Leinwand L.
The Journal of Biological Chemistry 2003 Aug; 278(33):31233.
Application：WB-Ti, Human, Mouse, Heart.
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Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation.
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