CSTF2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CSTF2 polyclonal antibody (Cat # PAB3760) in Jurkat cell line lysate (35 ug/lane). CSTF2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CSTF2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CSTF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CSTF2 polyclonal antibody (Cat # PAB3760) in Jurkat cell line lysate (35 ug/lane). CSTF2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution). -
Gene Info — CSTF2
Entrez GeneID
1478Protein Accession#
NP_001316;P33240Gene Name
CSTF2
Gene Alias
CstF-64
Gene Description
cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Omim ID
600368Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq
Other Designations
CF-1 64 kDa subunit|OTTHUMP00000024310|betaCstF-64 variant 2|cleavage stimulation factor subunit 2|cleavage stimulation factor, 64kDa subunit
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Interactome
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Publication Reference
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Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation.
Takagaki Y, Manley JL.
Molecular Cell 1998 Dec; 2(6):761.
Application：WB-Ce, WB-Tr, Chicken, DT40, DT40-64 cells.
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Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition.
Martincic K, Campbell R, Edwalds-Gilbert G, Souan L, Lotze MT, Milcarek C.
PNAS 1998 Sep; 95(19):11095.
Application：WB-Ce, Human, Mouse, 3T6, Splenic B, SKW 6.4, HeLa cells.
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The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.
Takagaki Y, Seipelt RL, Peterson ML, Manley JL.
Cell 1996 Nov; 87(5):941.
Application：IP, WB-Ce, WB-Tr, Chicken, Human, Mouse, DT40 cells, HeLa cells, Mouse B cells.
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Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation.
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