TLR2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with TLR2 polyclonal antibody (Cat # PAB3542).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with TLR2 polyclonal antibody (Cat # PAB3542), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TLR2 polyclonal antibody (Cat # PAB3542) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Flow Cytometry
Flow cytometric analysis of CEM cells using TLR2 polyclonal antibody (Cat # PAB3542)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TLR2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human TLR2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with TLR2 polyclonal antibody (Cat # PAB3542).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with TLR2 polyclonal antibody (Cat # PAB3542), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TLR2 polyclonal antibody (Cat # PAB3542) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
Flow cytometric analysis of CEM cells using TLR2 polyclonal antibody (Cat # PAB3542)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — TLR2
Entrez GeneID
7097Protein Accession#
O60603Gene Name
TLR2
Gene Alias
CD282, TIL4
Gene Description
toll-like receptor 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq
Other Designations
toll/interleukin 1 receptor-like 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkappa B signaling.
Sandor F, Latz E, Re F, Mandell L, Repik G, Golenbock DT, Espevik T, Kurt-Jones EA, Finberg RW.
International Journal of Cell Biology 2003 Sep; 162(6):1099.
Application：ELISA, Func, Human, HEK 293 cells, Human PBMCs.
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Cellular recognition of tri-/di-palmitoylated peptides is independent from a domain encompassing the N-terminal seven leucine-rich repeat (LRR)/LRR-like motifs of TLR2.
Meng G, Grabiec A, Vallon M, Ebe B, Hampel S, Bessler W, Wagner H, Kirschning CJ.
The Journal of Biological Chemistry 2003 Jul; 278(41):39822.
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Airway epithelia regulate expression of human beta-defensin 2 through Toll-like receptor 2.
Wang X, Zhang Z, Louboutin JP, Moser C, Weiner DJ, Wilson JM.
FASEB Journal 2003 Jul; 17(12):1727.
Application：IF, IHC-Fr, WB-Tr, Human, HEK 293 cells, Human lung tissues.
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Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkappa B signaling.
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