EPHA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The EPHA2 polyclonal antibody (Cat # PAB3003) is used in Western blot to detect EPHA2 in CHO cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody (Cat # PAB3003) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using EPHA2 polyclonal antibody (Cat # PAB3003)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHA2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHA2.
Host
Rabbit
Reactivity
Hamster, Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100 Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The EPHA2 polyclonal antibody (Cat # PAB3003) is used in Western blot to detect EPHA2 in CHO cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody (Cat # PAB3003) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using EPHA2 polyclonal antibody (Cat # PAB3003)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — EPHA2
Entrez GeneID
1969Protein Accession#
P29317Gene Name
EPHA2
Gene Alias
ECK
Gene Description
EPH receptor A2
Omim ID
176946Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
Other Designations
ephrin receptor EphA2|epithelial cell receptor protein tyrosine kinase|protein tyrosine kinase|receptor protein tyrosine kinase regulated by p53 and E2F-1|soluble EPHA2 variant 1
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Interactome
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Pathway
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Disease
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Publication Reference
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cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases.
Lindberg RA, Hunter T.
Molecular and Cellular Biology 1990 Dec; 10(12):6316.
Application：IHC-Fr, IP, KA, Human, Rat, A-431 cells, Lungs,Kidneys.
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cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases.
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