EARS2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EARS2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human EARS2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EARS2 polyclonal antibody (Cat # PAB2973) in HL-60 cell line lysates (35 ug/lane).EARS2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testisreacted with EARS2 polyclonal antibody (Cat # PAB2973), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EARS2
Entrez GeneID
124454Protein Accession#
NP_001077083;Q5JPH6Gene Name
EARS2
Gene Alias
KIAA1970, MSE1
Gene Description
glutamyl-tRNA synthetase 2, mitochondrial (putative)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
glutamate tRNA ligase 2, mitochondrial (putative)|glutamyl tRNA synthetase 2
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Interactome
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Pathway
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Publication Reference
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Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Giege R, Florentz C, Sissler M.
Biochemistry 2005 Mar; 44(12):4805.
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Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
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