CCNA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CCNA2 polyclonal antibody (Cat # PAB2867) in HL-60 cell line lysates (35 ug/lane). CCNA2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with CCNA2 polyclonal antibody (Cat # PAB2867), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using CCNA2 polyclonal antibody (Cat # PAB2867)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCNA2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CCNA2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CCNA2 polyclonal antibody (Cat # PAB2867) in HL-60 cell line lysates (35 ug/lane). CCNA2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with CCNA2 polyclonal antibody (Cat # PAB2867), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of HepG2 cells using CCNA2 polyclonal antibody (Cat # PAB2867)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CCNA2
Entrez GeneID
890Protein Accession#
NP_001228;P20248Gene Name
CCNA2
Gene Alias
CCN1, CCNA
Gene Description
cyclin A2
Omim ID
123835Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq
Other Designations
cyclin A
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Interactome
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Pathway
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Disease
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Publication Reference
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Neuronal pentraxin 1: a novel mediator of hypoxic-ischemic injury in neonatal brain.
Hossain MA, Russell JC, O'Brien R, Laterra J.
Journal of Neuroscience 2004 Apr; 24(17):4187.
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Neuronal pentraxin, a secreted protein with homology to acute phase proteins of the immune system.
Schlimgen AK, Helms JA, Vogel H, Perin MS.
Neuron 1995 Mar; 14(3):519.
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Neuronal pentraxin 1: a novel mediator of hypoxic-ischemic injury in neonatal brain.
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