RIOK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with RIOK1 polyclonal antibody (Cat # PAB2762).
Western Blot (Transfected lysate)
Western blot analysis of RIOK1 (arrow) using RIOK1 polyclonal antibody (Cat # PAB2762).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RIOK1 gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RIOK1 polyclonal antibody (Cat # PAB2762) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RIOK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RIOK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with RIOK1 polyclonal antibody (Cat # PAB2762).Western Blot (Transfected lysate)
Western blot analysis of RIOK1 (arrow) using RIOK1 polyclonal antibody (Cat # PAB2762).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RIOK1 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RIOK1 polyclonal antibody (Cat # PAB2762) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — RIOK1
Entrez GeneID
83732Protein Accession#
NP_113668;Q9BRS2Gene Name
RIOK1
Gene Alias
AD034, FLJ30006, MGC12903, bA288G3.1
Gene Description
RIO kinase 1 (yeast)
Gene Ontology
HyperlinkGene Summary
This gene includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000015999|RIO kinase 1|prematurely truncated due to insertion of intronic sequence
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Interactome
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Publication Reference
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K
Nature Genetics 2003 Dec; 36(1):40.
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
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