SGCD polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SGCD polycolnal antibody (Cat # PAB27590) (0.1ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SGCD polycolnal antibody (Cat # PAB27590) (3.8ug/ml) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SGCD.
Immunogen
A synthetic peptide corresponding to amino acids 245-257 at internal region of human SGCD.
Sequence
RLPHGSYTPTGTR
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Chicken, Dog, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3ug/ml)
Immunohistochemistry (3-5ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SGCD polycolnal antibody (Cat # PAB27590) (0.1ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SGCD polycolnal antibody (Cat # PAB27590) (3.8ug/ml) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Gene Info — SGCD
Entrez GeneID
6444Protein Accession#
NP_000328.2;NP_758447.1;NP_001121681.1Gene Name
SGCD
Gene Alias
35DAG, CMD1L, DAGD, MGC22567, SG-delta, SGCDP, SGD
Gene Description
sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq
Other Designations
35kD dystrophin-associated glycoprotein|delta-sarcoglycan|dystrophin associated glycoprotein, delta sarcoglycan|placental delta sarcoglycan|sarcoglycan, delta (35kD dystrophin-associated glycoprotein)
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Interactome
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Pathway
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Disease
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