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Last updated: 2014/4/14
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BIOtech
BULK-new

PHB polyclonal antibody

  • Catalog # : PAB2548
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PHB.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 189-219 at C-terminus of human PHB.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of PHB1 (arrow) using rabbit PHB polyclonal antibody (Cat # PAB2548). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB1 gene (Lane 2) (Origene Technologies).
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5245
  • Protein Accession#:
  • NP_002625;P35232
  • Gene Name:
  • PHB
  • Gene Alias:
  • PHB1
  • Gene Description:
  • prohibitin
  • Gene Summary:
  • Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq
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