APAF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of COLO 205 cell lysate with APAF1 polyclonal antibody (Cat # PAB24797).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using APAF1 polyclonal antibody (Cat # PAB24797).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of APAF1.
Immunogen
A synthetic peptide corresponding to APAF1.
Host
Rabbit
Theoretical MW (kDa)
27
Reactivity
Human
Specificity
APAF1 polyclonal antibody detects endogenous levels of APAF1 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of COLO 205 cell lysate with APAF1 polyclonal antibody (Cat # PAB24797).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using APAF1 polyclonal antibody (Cat # PAB24797).Immunofluorescence
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Gene Info — APAF1
Entrez GeneID
317Gene Name
APAF1
Gene Alias
CED4, DKFZp781B1145
Gene Description
apoptotic peptidase activating factor 1
Omim ID
602233Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
apoptotic protease activating factor 1
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Interactome
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Pathway
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Disease
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