ADAM17 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ADAM17 polyclonal antibody (Cat # PAB1888) in CEM cell line lysates (35 ug/lane). ADAM17 (arrow) was detected using the purified polyclonal antibody.
Flow Cytometry
Flow cytometric analysis of CEM cells using ADAM17 polyclonal antibody (Cat # PAB1888)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ADAM17.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ADAM17.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ADAM17 polyclonal antibody (Cat # PAB1888) in CEM cell line lysates (35 ug/lane). ADAM17 (arrow) was detected using the purified polyclonal antibody.Flow Cytometry
Flow cytometric analysis of CEM cells using ADAM17 polyclonal antibody (Cat # PAB1888)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ADAM17
Entrez GeneID
6868Protein Accession#
NP_003174;P78536Gene Name
ADAM17
Gene Alias
CD156b, MGC71942, TACE, cSVP
Gene Description
ADAM metallopeptidase domain 17
Omim ID
603639Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq
Other Designations
TNF-alpha converting enzyme|a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme)|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme
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Interactome
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Pathway
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Disease
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Publication Reference
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HDLs activate ADAM17-dependent shedding.
Tellier E, Canault M, Poggi M, Bonardo B, Nicolay A, Alessi MC, Nalbone G, Peiretti F.
Journal of Cellular Physiology 2008 Mar; 214(3):687.
Application：WB, Human, Human high-density lipoproteins.
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Involvement of NF-kappaB-mediated maturation of ADAM-17 in the invasion of oral squamous cell carcinoma.
Takamune Y, Ikebe T, Nagano O, Shinohara M.
Biochemical and Biophysical Research Communications 2008 Jan; 365(2):393.
Application：WB, Human, OSCC cells.
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HDLs activate ADAM17-dependent shedding.
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