ERCC1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ERCC1.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human ERCC1.
Sequence
DPGKDKEGVPQPS-C
Host
Goat
Theoretical MW (kDa)
38
Reactivity
Human
Specificity
This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ERCC1 polyclonal antibody (Cat # PAB18667) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ERCC1
Entrez GeneID
2067Protein Accession#
NP_973730.1;NP_001974.1Gene Name
ERCC1
Gene Alias
COFS4, UV20
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
excision repair cross-complementing 1|excision repair protein
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Interactome
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Pathway
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Disease
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