MYC polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with Forskolin (40 nM, 30 min), using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYC.
Immunogen
A synthetic peptide corresponding to human MYC.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MYC.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with Forskolin (40 nM, 30 min), using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MYC polyclonal antibody (Cat # PAB18101).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYC
Entrez GeneID
4609Protein Accession#
P01106Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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The Fbw7 tumor suppressor regulates glycogen synthase kinase 3 phosphorylation-dependent c-Myc protein degradation.
Welcker M, Orian A, Jin J, Grim JE, Harper JW, Eisenman RN, Clurman BE.
PNAS 2004 Jun; 101(24):9085.
Application：WB-Tr, Human, HeLa, HEK 293, HFF, U-2 OS cells.
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Phosphorylation by glycogen synthase kinase-3 controls c-myc proteolysis and subnuclear localization.
Gregory MA, Qi Y, Hann SR.
The Journal of Biological Chemistry 2003 Dec; 278(51):51606.
Application：IF, IP, WB, Monkey, COS-7 cells.
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The c-Myc transactivation domain is a direct modulator of apoptotic versus proliferative signals.
Chang DW, Claassen GF, Hann SR, Cole MD.
Molecular and Cellular Biology 2000 Jun; 20(12):4309.
Application：IP, WB, Rat, Rat fibroblasts.
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The Fbw7 tumor suppressor regulates glycogen synthase kinase 3 phosphorylation-dependent c-Myc protein degradation.
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