PIKFYVE polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using PIKFYVE polyclonal antibody (Cat # PAB17475).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using PIKFYVE polyclonal antibody (Cat # PAB17475).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIKFYVE.
Immunogen
A synthetic peptide corresponding to internal of human PIKFYVE.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total PIKFYVE protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using PIKFYVE polyclonal antibody (Cat # PAB17475).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using PIKFYVE polyclonal antibody (Cat # PAB17475).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIP5K3
Entrez GeneID
200576Protein Accession#
Q9Y2I7Gene Name
PIP5K3
Gene Alias
CFD, FAB1, KIAA0981, MGC40423, PIKFYVE, PIP5K
Gene Description
phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
Gene Ontology
HyperlinkGene Summary
PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization (Shisheva et al., 1999 [PubMed 9858586]).[supplied by OMIM
Other Designations
1-phosphatidylinositol-4-phosphate 5-kinase|FYVE finger-containing phosphoinositide kinase|OTTHUMP00000163872|PtdIns(4)P-5-kinase|phosphatidylinositol-3-phosphate 5-kinase type III|phosphatidylinositol-4-phosphate 5-kinase, type III
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Interactome
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Pathway
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Disease
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Publication Reference
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Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site.
Cuesta-Geijo MÁ, Barrado-Gil L, Galindo I, Muñoz-Moreno R, Alonso C.
Viruses 2017 Jun; 9(6):E133.
Application：WB-Ce, Monkey, Vero cells.
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Redistribution of Endosomal Membranes to the African Swine Fever Virus Replication Site.
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