GAPDH polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of GAPDH expression in 293 (A) and HeLa (B) whole cell lysates and mouse kidney tissue extract (C) using GAPDH polyclonal antibody (Cat # PAB17013).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant GAPDH.
Immunogen
Recombinant protein corresponding to human GAPDH.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:4000)
Immunofluorescence (1:100-1:400)
Immunohistochemistry (1:100-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of GAPDH expression in 293 (A) and HeLa (B) whole cell lysates and mouse kidney tissue extract (C) using GAPDH polyclonal antibody (Cat # PAB17013).Immunohistochemistry
Immunofluorescence
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Gene Info — GAPDH
Entrez GeneID
2597Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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TZAP overexpression induces telomere dysfunction and ALT-like activity in ATRX/DAXX-deficient cells.
Sara Priego Moreno, Javier Miralles Fusté, Melanie Kaiser, Julia Su Zhou Li, Joe Nassour, Candy Haggblom, Eros Lazzerini Denchi, Jan Karlseder.
iScience 2023 Mar; 26(4):106405.
Application：WB-Ce, Human, U-2 OS cells, GM847, HeLa 1.2.11 cells, HT-1080 cells.
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Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis.
Joe Nassour, Lucia Gutierrez Aguiar, Adriana Correia, Tobias T Schmidt, Laura Mainz, Sara Przetocka, Candy Haggblom, Nimesha Tadepalle, April Williams, Maxim N Shokhirev, Semih C Akincilar, Vinay Tergaonkar, Gerald S Shadel, Jan Karlseder.
Nature 2023 Feb; 614(7949):767.
Application：WB-Tr, Human, IMR90 cells.
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Autophagic cell death restricts chromosomal instability during replicative crisis.
Nassour J, Radford R, Correia A, Fusté JM, Schoell B, Jauch A, Shaw RJ, Karlseder J.
Nature 2019 Jan; 565(7741):659.
Application：WB, Human, Human mammary epithelial cells.
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Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP.
Cummings J, Ward TH, LaCasse E, Lefebvre C, St-Jean M, Durkin J, Ranson M, Dive C.
British Journal of Cancer 2005 Feb; 92(3):532.
Application：WB-Tr, Human, MDA-MB-231/X-G4 cells.
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TZAP overexpression induces telomere dysfunction and ALT-like activity in ATRX/DAXX-deficient cells.
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