AMFR polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of AMFR (arrow) using AMFR polyclonal antibody (Cat # PAB1684). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with AMFR polyclonal antibody (Cat # PAB1684) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using AMFR polyclonal antibody (Cat # PAB1684)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AMFR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 571-601 of human AMFR.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of AMFR (arrow) using AMFR polyclonal antibody (Cat # PAB1684). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with AMFR polyclonal antibody (Cat # PAB1684) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of HepG2 cells using AMFR polyclonal antibody (Cat # PAB1684)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — AMFR
Entrez GeneID
267Protein Accession#
P26442Gene Name
AMFR
Gene Alias
GP78, RNF45
Gene Description
autocrine motility factor receptor
Omim ID
603243Gene Ontology
HyperlinkGene Summary
Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.
Huang B, Xie Y, Raz A.
Biochemical and Biophysical Research Communications 1995 Jul; 212(3):727.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor.
Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A.
The Journal of Biological Chemistry 1991 Jul; 266(20):13442.
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Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.
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