RBM3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
RBM3 polyclonal antibody (Cat # PAB15658) (0.01 ug/mL) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of RBM3.
Immunogen
A synthetic peptide corresponding to human RBM3.
Sequence
C-DRYSGGNYRDNYDN
Host
Goat
Theoretical MW (kDa)
17.2
Reactivity
Human
Specificity
Approximately 17 KDa band observed in lysates of cell line HepG2 (calculated MW of 17.2 KDa according to NP_006734.1).
An additional band of unknown identity was also consistently observed at 48 KDa. This band was successfully blocked by incubation with the immunizing peptide.Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RBM3 polyclonal antibody (Cat # PAB15658) (0.01 ug/mL) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RBM3
Entrez GeneID
5935Protein Accession#
NP_006734.1Gene Name
RBM3
Gene Alias
IS1-RNPL, RNPL
Gene Description
RNA binding motif (RNP1, RRM) protein 3
Omim ID
300027Gene Ontology
HyperlinkGene Summary
This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq
Other Designations
OTTHUMP00000025800|OTTHUMP00000025802|RNA binding motif protein 3
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Interactome
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Publication Reference
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Translation regulatory factor RBM3 is a proto-oncogene that prevents mitotic catastrophe.
Sureban SM, Ramalingam S, Natarajan G, May R, Subramaniam D, Bishnupuri KS, Morrison AR, Dieckgraefe BK, Brackett DJ, Postier RG, Houchen CW, Anant S.
Oncogene 2008 Jul; 27(33):4544.
Application：IHC, WB-Ti, WB-Tr, Human, Human Breast, colon, lung, ovary, pancreas, prostate cancer, HCT-116 cells.
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Translation regulatory factor RBM3 is a proto-oncogene that prevents mitotic catastrophe.
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