AOF2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot was performed using respectively 150 ug (Lane 1) or 50 ug (Lane 2) nuclear extracts from HeLa cells and AOF2 polyclonal antibody (Cat # PAB14134) diluted 1 : 1,000.
Western Blot (Transfected lysate)
293T cells were transiently transfected with an expression vector for HA-tagged AOF2 (Lanes 1) or with an empty vector (Lanes 2).
A : Whole cell extracts were analysed by Western blot using AOF2 polyclonal antibody (Cat # PAB14134), diluted 1 : 1,000.
B. Whole cell extracts were immunoprecipitated with 2 ug of AOF2 polyclonal antibody (Cat # PAB14134). Immunoprecipitates were analysed by Western blot as described above.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of AOF2 polyclonal antibody (Cat # PAB14134).
The plates were coated with the peptide used for immunization of the rabbit.
By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 2,500.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AOF2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human AOF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100)
Western Blot (1:1000)
Immunoprecipitation (2 ug/IP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot was performed using respectively 150 ug (Lane 1) or 50 ug (Lane 2) nuclear extracts from HeLa cells and AOF2 polyclonal antibody (Cat # PAB14134) diluted 1 : 1,000.Western Blot (Transfected lysate)
293T cells were transiently transfected with an expression vector for HA-tagged AOF2 (Lanes 1) or with an empty vector (Lanes 2).
A : Whole cell extracts were analysed by Western blot using AOF2 polyclonal antibody (Cat # PAB14134), diluted 1 : 1,000.
B. Whole cell extracts were immunoprecipitated with 2 ug of AOF2 polyclonal antibody (Cat # PAB14134). Immunoprecipitates were analysed by Western blot as described above.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of AOF2 polyclonal antibody (Cat # PAB14134).
The plates were coated with the peptide used for immunization of the rabbit.
By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 2,500. -
Gene Info — AOF2
Entrez GeneID
23028Protein Accession#
O60341Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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