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Last updated: 2014/4/14
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BIOtech
BULK-new

AOF2 polyclonal antibody

  • Catalog # : PAB14121
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of AOF2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human AOF2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:200-1:1000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 40 ug) and AOF2 polyclonal antibody (Cat # PAB14121) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of AOF2 polyclonal antibody (Cat # PAB14121), crude serum and Flow Through in antigen coated wells.
    By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 20,000.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • AOF2
  • Gene Alias:
  • BHC110,KDM1,KIAA0601,LSD1
  • Gene Description:
  • amine oxidase (flavin containing) domain 2
  • Gene Summary:
  • This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • BRAF35-HDAC complex protein BHC110,FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit,lysine (K)-specific demethylase 1,lysine-specific histone demethylase 1
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