Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 40 ug) and AOF2 polyclonal antibody (Cat # PAB14121) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of AOF2 polyclonal antibody (Cat # PAB14121), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 20,000.
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
BRAF35-HDAC complex protein BHC110,FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit,lysine (K)-specific demethylase 1,lysine-specific histone demethylase 1