NANOG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot with NANOG polyclonal antibody (Cat # PAB13694) and ACTB monoclonal antibody. Each lane using 20 ug protein.
Immunofluorescence
Immunofluorescence with NANOG polyclonal antibody (Cat # PAB13694). Human embryonic stem cells, grown on mouse embryonic fibroblasts. Nuclear staining by DAPI.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NANOG.
Immunogen
A synthetic peptide corresponding to human NANOG.
Host
Rabbit
Reactivity
Human
Specificity
Recognizes human Nanog.
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:4000)
Immunocytochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot with NANOG polyclonal antibody (Cat # PAB13694) and ACTB monoclonal antibody. Each lane using 20 ug protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence with NANOG polyclonal antibody (Cat # PAB13694). Human embryonic stem cells, grown on mouse embryonic fibroblasts. Nuclear staining by DAPI. -
Gene Info — NANOG
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Interactome
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Publication Reference
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Nanog expression in mouse germ cell development.
Hansen PB, Jensen BL, Yamaguchi S, Andreasen D, Kimura H, Friis UG, Tada M, Skott O, Nakatsuji N, Tada T.
Gene Expression Patterns 2005 Jun; 5(5):639.
Application：IF, IHC-Fr, Mouse, Mouse embryos.
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Pluripotential competence of cells associated with Nanog activity.
Hatano SY, Tada M, Kimura H, Yamaguchi S, Kono T, Hussain SP, Nakano T, Hollstein MH, Suemori H, Harris CC, Nakatsuji N, Tada T.
Mechanisms of Development 2005 Jan; 122(1):67.
Application：IF, WB, Human, Mouse, Embryonic stem cells, NIH/3T3 cells, Thymocytes.
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Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells.
Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, Smith A.
Cell 2003 May; 113(5):643.
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Nanog expression in mouse germ cell development.
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