BLNK polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using BLNK polyclonal antibody (Cat # PAB13541).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant BLNK.
Immunogen
Recombinant protein corresponding to amino acids 171-356 of human BLNK.
Host
Rabbit
Theoretical MW (kDa)
68, 70
Reactivity
Human, Mouse
Specificity
This antibody reacts with mouse BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 KDa in SDS PAGE (alternatively spliced forms of human SLP65).
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using BLNK polyclonal antibody (Cat # PAB13541). -
Gene Info — BLNK
Entrez GeneID
29760Gene Name
BLNK
Gene Alias
BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65
Gene Description
B-cell linker
Omim ID
604515Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
B cell linker protein|B-cell adapter containing a SH2 domain protein|B-cell adapter containing a Src homology 2 domain protein|OTTHUMP00000020167|Src homology 2 domain-containing leukocyte protein of 65 kDa
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Interactome
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Pathway
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Disease
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Publication Reference
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SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond.
Koretzky GA, Abtahian F, Silverman MA.
Nature Reviews. Immunology 2006 Jan; 6(1):67.
Application：WB-Tr, Chicken, DT40 cells.
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Bruton's tyrosine kinase cooperates with the B cell linker protein SLP-65 as a tumor suppressor in Pre-B cells.
Kersseboom R, Middendorp S, Dingjan GM, Dahlenborg K, Reth M, Jumaa H, Hendriks RW.
The Journal of Experimental Medicine 2003 Jul; 198(1):91.
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The absence of SLP65 and Btk blocks B cell development at the preB cell receptor-positive stage.
Jumaa H, Mitterer M, Reth M, Nielsen PJ.
European Journal of Immunology 2001 Jul; 31(7):2164.
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SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond.
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