AGAP2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AGAP2.
Immunogen
A synthetic peptide corresponding to C-terminus 15 amino acids of human AGAP2.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of AGAP2 in mouse brain tissue lysates with AGAP2 polyclonal antibody (Cat # PAB12921) at (A) 0.5, (B) 1, and (C) 2 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of AGAP2 in mouse brain tissue with AGAP2 polyclonal antibody (Cat # PAB12921) at 10 ug/mL . -
Gene Info — AGAP2
Entrez GeneID
116986Protein Accession#
AAM97540Gene Name
AGAP2
Gene Alias
CENTG1, FLJ16430, GGAP2, KIAA0167, PIKE
Gene Description
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Omim ID
605476Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2|GTP-binding and GTPase activating protein 2|centaurin, gamma 1|phosphoinositide 3-kinase enhancer
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Pathway
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Disease
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Publication Reference
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PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.
Ahn JY, Rong R, Kroll TG, Van Meir EG, Snyder SH, Ye K.
The Journal of Biological Chemistry 2004 Apr; 279(16):16441.
Application:IF, WB-Ce, Human, NGP-127, CRL-2061, U87MG, LN487, LN382, LNZ308 cells.
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PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis.
Rong R, Ahn JY, Huang H, Nagata E, Kalman D, Kapp JA, Tu J, Worley PF, Snyder SH, Ye K.
Nature Neuroscience 2003 Nov; 6(11):1153.
Application:IF, WB-Ti, Rat, Rat brains.
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Cytohesins and centaurins: mediators of PI 3-kinase-regulated Arf signaling.
Jackson TR, Kearns BG, Theibert AB.
Trends in Biochemical Sciences 2000 Oct; 25(10):489.
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PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.
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