MYC tag polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
The cell lysate of 293 with over-expressed MYC tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane.
Followed by an immune-blotting with MYC tag polyclonal antibody (Cat # PAB12716) at 1 : 1000.
Lane 2 was pre-incubated by immunizing peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYC tag.
Immunogen
A synthetic peptide corresponding to amino acids 408-452 of human MYC tag.
Sequence
EQKLISEEDL
Host
Rabbit
Specificity
This antibody recognizes MYC tagged fusion proteins.
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
The cell lysate of 293 with over-expressed MYC tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane.
Followed by an immune-blotting with MYC tag polyclonal antibody (Cat # PAB12716) at 1 : 1000.
Lane 2 was pre-incubated by immunizing peptide.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYC
Entrez GeneID
4609Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Paradoxical effects of DNA tumor virus oncogenes on epithelium-derived tumor cell fate during tumor progression and chemotherapy response.
Jiang He, Liyu Liu, Feiyu Tang, You Zhou, Huan Liu, Can Lu, Deyun Feng, Hong Zhu, Yitao Mao, Zhi Li, Lu Zhang, Yuemei Duan, Zhi Xiao, Musheng Zeng, Liang Weng, Lun-Quan Sun.
Signal Transduction and Targeted Therapy 2021 Nov; 6(1):408.
Application：WB-Tr, Human, HEK 293T (Bosc 23) cells.
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The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
Campbell AM, Kessler PD, Fambrough DM.
The Journal of Biological Chemistry 1992 May; 267(13):9321.
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Paradoxical effects of DNA tumor virus oncogenes on epithelium-derived tumor cell fate during tumor progression and chemotherapy response.
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