ABHD5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of ABHD5 in MCF-7 cell lysates using ABHD5 polyclonal antibody (Cat # PAB12500).
Western Blot (Transfected lysate)
Western blot analysis of ABHD5 in ABHD5 overexpressed lysates using ABHD5 polyclonal antibody (Cat # PAB12500).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ABHD5.
Immunogen
A synthetic peptide corresponding to amino acids 200-300 of human ABHD5.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of ABHD5 in MCF-7 cell lysates using ABHD5 polyclonal antibody (Cat # PAB12500).Western Blot (Transfected lysate)
Western blot analysis of ABHD5 in ABHD5 overexpressed lysates using ABHD5 polyclonal antibody (Cat # PAB12500). -
Gene Info — ABHD5
Entrez GeneID
51099Protein Accession#
Q8WTS1Gene Name
ABHD5
Gene Alias
CDS, CGI58, IECN2, MGC8731, NCIE2
Gene Description
abhydrolase domain containing 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.
Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, Zechner R, Kiens B.
American Journal of Physiology. Endocrinology and Metabolism 2009 Mar; 296(3):E445.
Application：WB-Ti, Human, Mouse, Human vastus lateralis muscle, mouse white adipose tissue.
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Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes.
Subramanian V, Rothenberg A, Gomez C, Cohen AW, Garcia A, Bhattacharyya S, Shapiro L, Dolios G, Wang R, Lisanti MP, Brasaemle DL.
The Journal of Biological Chemistry 2004 Oct; 279(40):42062.
Application：IF, Mouse, 3T3-L1 cells.
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CGI-58 interacts with perilipin and is localized to lipid droplets. Possible involvement of CGI-58 mislocalization in Chanarin-Dorfman syndrome.
Yamaguchi T, Omatsu N, Matsushita S, Osumi T.
The Journal of Biological Chemistry 2004 Jul; 279(29):30490.
Application：IF, WB-Ti, Mouse, Rat, Rat adipose tissue, 3T3-L1 cells.
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Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.
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