NES polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of NES in human fetal temporal lobe tissue lysates using NES polyclonal antibody (30 ug) (Cat # PAB12375). Lane 1 : 0.5 ug/mL . Lane 2 : 2 ug/mL . ECL : 1 second exposure.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant NES.
Immunogen
Recombinant protein corresponding to amino acids 1464-1614 of human NES.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human nestin.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NES in human fetal temporal lobe tissue lysates using NES polyclonal antibody (30 ug) (Cat # PAB12375). Lane 1 : 0.5 ug/mL . Lane 2 : 2 ug/mL . ECL : 1 second exposure. -
Gene Info — NES
Entrez GeneID
10763Protein Accession#
P48681Gene Name
NES
Gene Alias
FLJ21841, Nbla00170
Gene Description
nestin
Omim ID
600915Gene Ontology
HyperlinkGene Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
Other Designations
OTTHUMP00000032198
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Interactome
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Disease
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Publication Reference
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Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody.
Messam CA, Hou J, Major EO.
Experimental Neurology 2000 Feb; 161(2):585.
Application：IF, WB-Ce, WB-Ti, Human, Rat, U251, U373, SVG cells, Brain, HFBCs.
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Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody.
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