ALPL polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ALPL.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human ALPL.
Host
Rabbit
Theoretical MW (kDa)
57.3
Reactivity
Human, Mouse, Rat
Specificity
Different from the related rat and mouse sequence by two amino acids.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of ALPL expression from HeLa cell lyate with ALPL polyclonal antibody (Cat # PAB12279).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ALPL on formalin fixed, paraffin embedded rat small intestine in cytoplasm DAB chromogenic reaction. Using ALPL polyclonal antibody (Cat # PAB12279). -
Gene Info — ALPL
Entrez GeneID
249GeneBank Accession#
AB011406Protein Accession#
BAA32129Gene Name
ALPL
Gene Alias
AP-TNAP, FLJ40094, FLJ93059, HOPS, MGC161443, MGC167935, TNAP, TNSALP
Gene Description
alkaline phosphatase, liver/bone/kidney
Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000002971|OTTHUMP00000002972|alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphomonoesterase|glycerophosphatase|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP
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Interactome
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Pathway
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Disease
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Publication Reference
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Matrix vesicles promote bone repair after a femoral bone defect in mice.
Yuya Mizukami, Naoyuki Kawao, Yoshimasa Takafuji, Takashi Ohira, Kiyotaka Okada, Jun-Ichiro Jo, Yasuhiko Tabata, Hiroshi Kaji.
PLoS One 2023 Apr; 18(4):e0284258.
Application:IF, Mouse, Mouse femur.
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Renal failure suppresses muscle irisin expression, and irisin blunts cortical bone loss in mice.
Naoyuki Kawao, Miku Kawaguchi, Takashi Ohira, Hiroki Ehara, Yuya Mizukami, Yoshimasa Takafuji, Hiroshi Kaji.
Journal of Cachexia, Sarcopenia and Muscle 2022 Feb; 13(1):758.
Application:IHC, Mouse, Mouse bone.
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Plasminogen activator inhibitor-1 is involved in impaired bone repair associated with diabetes in female mice.
Mao L, Kawao N, Tamura Y, Okumoto K, Okada K, Yano M, Matsuo O, Kaji H.
PLoS One 2014 Mar; 9(3):e92686.
Application:IHC, Mouse, Bone.
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The Extracellular cAMP-Adenosine Pathway Regulates Expression of Renal D1 Dopamine Receptors in Diabetic Rats.
Kuzhikandathil EV, Clark L, Li Y.
The Journal of Biological Chemistry 2011 Sep; 286(37):32454.
Application:WB, Mouse, Mouse kidneys.
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Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5'-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study.
K N Fedde, M P Whyte.
American Journal of Human Genetics 1990 Nov; 47(5):767.
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A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia.
Weiss MJ, Cole DE, Ray K, Whyte MP, Lafferty MA, Mulivor RA, Harris H.
PNAS 1988 Oct; 85(20):7666.
Application:ICC, Mouse , NIH/3T3 cells.
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Matrix vesicles promote bone repair after a femoral bone defect in mice.
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