Clock polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Clock.
Immunogen
A mixture of two synthetic peptides (conjugated with albumin) corresponding to mouse Clock.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to CLOCK.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Western Blot
Immunoprecipitation
Lanes 1-3 contained Clock polyclonal antibody (Cat # PAB12145) immunoprecipitated DNA (15, 10 and 5 uL of antisera added respectively to the 900 uL of sonicated chromatin sample in the ChIP assay). -
Gene Info — Clock
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Publication Reference
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Circadian Transcription. Thinking outside the E-Box.
Munoz E, Brewer M, Baler R.
The Journal of Biological Chemistry 2002 Sep; 277(39):36009.
Application:WB-Tr, Mouse, NIH-3T3 cells.
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Photic induction of Period gene expression is reduced in Clock mutant mice.
Shearman LP, Weaver DR.
Neuroreport 1999 Feb; 10(3):613.
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A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock.
Jin X, Shearman LP, Weaver DR, Zylka MJ, de Vries GJ, Reppert SM.
Cell 1999 Jan; 96(1):57.
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Circadian rhythms. An end in the beginning.
Dunlap J.
Science 1998 Jun; 280(5369):1548.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors.
Hogenesch JB, Gu YZ, Jain S, Bradfield CA.
PNAS 1998 May; 95(10):5474.
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Circadian Transcription. Thinking outside the E-Box.
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