S100A9 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
S100A9 polyclonal antibody (Cat # PAB11470) (0.5 ug/mL) staining of human peripheral blood mononucleocytes (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
S100A9 polyclonal antibody (Cat # PAB11470) (2.5 ug/mL) staining of paraffin embedded human lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of S100A9.
Immunogen
A synthetic peptide corresponding to human S100A9.
Sequence
C-DTNADKQLSFEEF
Host
Goat
Theoretical MW (kDa)
13.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
S100A9 polyclonal antibody (Cat # PAB11470) (0.5 ug/mL) staining of human peripheral blood mononucleocytes (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
S100A9 polyclonal antibody (Cat # PAB11470) (2.5 ug/mL) staining of paraffin embedded human lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — S100A9
Entrez GeneID
6280Protein Accession#
NP_002956.1Gene Name
S100A9
Gene Alias
60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14
Gene Description
S100 calcium binding protein A9
Omim ID
123886Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq
Other Designations
OTTHUMP00000015331|S100 calcium-binding protein A9|S100 calcium-binding protein A9 (calgranulin B)|calgranulin B
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Interactome
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Disease
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Publication Reference
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Alarmins in Frozen Shoulder: A Molecular Association Between Inflammation and Pain.
Cher JZB, Akbar M, Kitson S, Crowe LAN, Garcia-Melchor E, Hannah SC, McLean M, Fazzi UG, Kerr SC, Murrell GAC, L Millar N.
The American Journal of Sports Medicine 2017 Nov; 1:3635465177.
Application：IHC-P, Human, Shoulder capsule samples from patients with primary frozen shoulders who were receiving arthroscopic capsular releases.
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Methods for predicting rheumatoid arthritis treatment response.
Olivier Vittecoq, Thierry Lequerre, Pascal Cosette, Olivier Boyer, Xavier Le Loet, Julie Hardouin, Antoine Obry.
United States Patent Application Publication 2016 May; [Epub].
Application：ELISA, Human, Serum.
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Metal chelation and inhibition of bacterial growth in tissue abscesses.
Corbin BD, Seeley EH, Raab A, Feldmann J, Miller MR, Torres VJ, Anderson KL, Dattilo BM, Dunman PM, Gerads R, Caprioli RM, Nacken W, Chazin WJ, Skaar EP.
Science 2008 Feb; 319(5865):962.
Application：IHC, Mouse , Mouse kidney.
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S100A8 and S100A9 in human arterial wall. Implications for atherogenesis.
McCormick MM, Rahimi F, Bobryshev YV, Gaus K, Zreiqat H, Cai H, Lord RS, Geczy CL.
The Journal of Biological Chemistry 2005 Dec; 280(50):41521.
Application：IHC, WB, Human, Aortic, Carotid specimens.
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Alarmins in Frozen Shoulder: A Molecular Association Between Inflammation and Pain.
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