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BIOtech
BULK-new

ALDOA polyclonal antibody

  • Catalog # : PAB11351
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against native ALDOA.
  • Immunogen:
  • Native purified ALDOA from rabbit muscle.
  • Host:
  • Goat
  • Reactivity:
  • Rabbit
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Native Purified Protein.
  • Recommend Usage:
  • Sandwich ELISA (1:10000-1:40000)
    Immunoprecipitation (5 ul)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • IgG purified ALDOA polyclonal antibody (Cat # PAB11351) to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot.
    A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE.
    The gel was transferred to nitrocellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk.
    Color was allowed to develop using Super Signal West Pico Chemiluminescent Substrate (PIERCE).
    This antibody clearly detects a band at ~41 KDa consistent with human aldolase.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • ALDOA
  • Gene Alias:
  • -
  • Gene Description:
  • aldolase A
  • Other Designations:
  • -
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