ALDOA polyclonal antibody
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More Files
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Western Blot (Cell lysate)
IgG purified ALDOA polyclonal antibody (Cat # PAB11351) to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot.
A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE.
The gel was transferred to nitrocellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk.
Color was allowed to develop using Super Signal West Pico Chemiluminescent Substrate (PIERCE).
This antibody clearly detects a band at ~41 KDa consistent with human aldolase.
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Specification
Product Description
Goat polyclonal antibody raised against native ALDOA.
Immunogen
Native purified ALDOA from rabbit muscle.
Host
Goat
Reactivity
Rabbit
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Sandwich ELISA (1:10000-1:40000)
Immunoprecipitation (5 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
IgG purified ALDOA polyclonal antibody (Cat # PAB11351) to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot.
A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE.
The gel was transferred to nitrocellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk.
Color was allowed to develop using Super Signal West Pico Chemiluminescent Substrate (PIERCE).
This antibody clearly detects a band at ~41 KDa consistent with human aldolase.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ALDOA
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