Rabbit polyclonal antibody raised against synthetic peptide of AKT1/AKT2.
A synthetic peptide corresponding to N-terminus of human AKT1/AKT2.
Theoretical MW (kDa):
This antibody recognizes 60 KDa for AKT1 and 56 KDa for AKT2.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (1:50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (Formalin/PFA-fixed paraffin-embedded sections) (1:50) The optimal working dilution should be determined by the end user.
In 10 mM PBS, pH 7.4 (1% BSA, 0.1% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta