ANAPC10 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 in various cell extracts.
This antibody clearly detects a ~26 KDa band corresponding to human ANAPC10 (predicted molecular weight is 21 KDa).
All lanes contain 20 ug of lysate or extract as follows : lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A-431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.
Exposure time was 4 min.
Western Blot (Cell lysate)
Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 by western blot.
Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.
Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1 : 2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.
Exposure time was 5 min.
The expected molecular weight of human ANAPC10 is 21 KDa.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ANAPC10.
Immunogen
A synthetic peptide corresponding to N-terminus of human ANAPC10.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:80000)
Western Blot (1:500-1:1500)
Immunohistochemistry (1:250-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 in various cell extracts.
This antibody clearly detects a ~26 KDa band corresponding to human ANAPC10 (predicted molecular weight is 21 KDa).
All lanes contain 20 ug of lysate or extract as follows : lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A-431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.
Exposure time was 4 min.Western Blot (Cell lysate)
Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 by western blot.
Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.
Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1 : 2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.
Exposure time was 5 min.
The expected molecular weight of human ANAPC10 is 21 KDa.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ANAPC10
Entrez GeneID
10393Protein Accession#
NP_055700.1Gene Name
ANAPC10
Gene Alias
APC10, DKFZp564L0562, DOC1
Gene Description
anaphase promoting complex subunit 10
Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Publication Reference
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Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition.
Passmore LA, McCormack EA, Au SW, Paul A, Willison KR, Harper JW, Barford D.
The EMBO Journal 2003 Feb; 22(4):786.
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Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p.
Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D.
The Journal of Cell Biology 2001 Jul; 154(1):85.
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Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex.
Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM.
The Journal of Biological Chemistry 1999 May; 274(20):14500.
Application：WB-Ce, Human, HeLa cells.
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Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition.
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