ATG7 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100 (ECL) .
Immunofluorescence
Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase (MPO) appearing in green. Using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG7.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to C-terminus of human ATG7.
Sequence
DSTRDRTLDQQC
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Purity
Whole serum
Recommend Usage
ELISA
Immunofluorescence (1:100)
Western blot (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at 4°C.
After reconstitution with 100 uL of sterile water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100 (ECL) .Immunofluorescence
Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase (MPO) appearing in green. Using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ATG7
Entrez GeneID
10533Protein Accession#
O95352;Q9D906;Q641Y5;XP_001087808;XP_533740Gene Name
ATG7
Gene Alias
APG7-LIKE, APG7L, DKFZp434N0735, GSA7
Gene Description
ATG7 autophagy related 7 homolog (S. cerevisiae)
Omim ID
608760Gene Ontology
HyperlinkGene Summary
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq
Other Designations
APG7 autophagy 7-like|ubiquitin activating enzyme E1-like protein
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Polyubiquitination of p62/SQSTM1 is a prerequisite for Fas/CD95 aggregation to promote caspase-dependent apoptosis in cadmium-exposed mouse monocyte RAW264.7 cells.
Jung KT, Oh SH.
Scientific Reports 2019 Aug; 9(1):12240.
Application：WB-Tr, Mouse, Raw 264.7 cells.
-
Polyubiquitination of p62/SQSTM1 is a prerequisite for Fas/CD95 aggregation to promote caspase-dependent apoptosis in cadmium-exposed mouse monocyte RAW264.7 cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com