ATG10 polyclonal antibody
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Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody (Cat # PAB0732) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG10.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to C-terminus of human ATG10.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:100-1:1000)
Immunofluorescence (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody (Cat # PAB0732) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. -
Gene Info — ATG10
Entrez GeneID
83734Protein Accession#
Q9H0Y0Gene Name
ATG10
Gene Alias
APG10, APG10L, DKFZp586I0418, FLJ13954, pp12616
Gene Description
ATG10 autophagy related 10 homolog (S. cerevisiae)
Omim ID
610800Gene Ontology
HyperlinkGene Summary
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
Other Designations
APG10 autophagy 10-like
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Interactome
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Disease
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