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Last updated: 2017/11/19
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BDNF polyclonal antibody

  • Catalog # : PAB0700
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of BDNF.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 129-138 human BDNF.
  • Sequence:
  • HSDPARRGEL
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Rat
  • Specificity:
  • Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis.
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Immunohistochemistry (1:1000-1:2000)
    ELISA (1:1000-1:2000)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from whole serum.
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 100 uL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot (Tissue lysate) analysis of BDNF expression in human brain.
    Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in human brain.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot (Cell lysate) analysis of BDNF expression of SH-SY5Y cell lysates.
    Monomeric BDNF at 14 kDa in all samples, as well as monomeric proBDNF at 32 kDa in cell lysates prepared in RIPA buffer.
  • Western Blot
  • Western Blot
  • Western blot analysis of BDNF expression in human serum.
    Monomeric BDNF at 14 kDa in all samples, and BDNF isoform at 18 kDa in human serum.
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 627
  • Gene Name:
  • BDNF
  • Gene Alias:
  • MGC34632
  • Gene Description:
  • brain-derived neurotrophic factor
  • Gene Summary:
  • The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
  • Other Designations:
  • neurotrophin
  • Related Disease
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