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Last updated: 2014/4/21
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BIOtech
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YES1 (phospho Y527) polyclonal antibody

  • Catalog # : PAB0634
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of YES1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y537 of human YES1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Dot Blot (1:500)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Jurkat cell lysate with YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634).
    40 ug of lysate loaded, ECL detection; Lane 1, 2.5 ug/ml; Lane 2, 1 ug/ml; Lane 3, 0.62 ug/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634) on nitrocellulose membrane. 50 ng of phospho-peptide or non phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5 ug per mL.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7525
  • Protein Accession#:
  • NP_005424;P07947
  • Gene Name:
  • YES1
  • Gene Alias:
  • HsT441,P61-YES,Yes,c-yes
  • Gene Description:
  • v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
  • Gene Summary:
  • This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000162194,Yamaguchi sarcoma oncogene,cellular yes-1 protein,proto-oncogene tyrosine-protein kinase YES,viral oncogene yes-1 homolog 1
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