The FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471) is used in Western blot to detect FGFR in mouse liver tissue lysate. FGFR (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of FGFR1 (arrow) using FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471). 293 cell lysates (2 &mciro;g/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGFR1 (phospho Y653/654) polyclonal antibody (Cat # PAB0471) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq