Western blot analysis Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 6000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 500) with Texas Red (Red).
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq