ACTB monoclonal antibody, clone G043
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ACTB in 293 cells. 20 ug of total lysate was bloted with ACTB monoclonal antibody, clone G043 (Cat # MAB8182).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide corresponding to N-terminus of ACTB.
Host
Mouse
Reactivity
Bovine, Carp, Cat, Chicken, Dog, Guinea pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:4000)
Immunofluorescence (1:100-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACTB in 293 cells. 20 ug of total lysate was bloted with ACTB monoclonal antibody, clone G043 (Cat # MAB8182).Immunofluorescence
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Publication Reference
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Nebulin regulates the assembly and lengths of the thin filaments in striated muscle.
McElhinny AS, Schwach C, Valichnac M, Mount-Patrick S, Gregorio CC.
The Journal of Cell Biology 2005 Sep; 170(6):947.
Application：IF, Rat, Rat cardiomyocytes.
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bPAK-interacting exchange factor may regulate actin cytoskeleton through interaction with actin.
Lee CS, Kim KY, Im JB, Choi JW, Kim HK, Park JS, Shin EY, Kim SR, Kim EG.
Experimental & Molecular Medicine 2004 Dec; 36(6):582.
Application：IP, WB, Rat, PC-12 cells.
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Nebulin regulates the assembly and lengths of the thin filaments in striated muscle.
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