The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries.
Protein G purification
Western Blot (1:250) Flow Cytometry (1 ug per million cells) Immunocytochemistry/Immunofluorescence (1:100-1:1000) Immunohistochemistry (1:250) Immunoprecipitation (1:10-1:500) The optimal working dilution should be determined by the end user.
In PBS (0.02% sodium azide)
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100). Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles. (A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells. (B) Cd34 expression in a murine telogen hair follicle. (C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C. Scale bars: 100 mum.