B2M monoclonal antibody, clone B2M-01 (PE)
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using B2M monoclonal antibody, clone B2M-01 (Cat # MAB0899).
Lane 1 : RAJI (human Burkitt lymphoma cell line).
Lane 2 : EL4 (mouse lymphoblastic lymphoma cell line).
Lane 3 : U937 (human histiocytic lymphoma cell line).
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Specification
Product Description
Mouse monoclonal antibody raised against B2M.
Immunogen
Purified isolated human B2M.
Host
Mouse
Theoretical MW (kDa)
12
Reactivity
Human
Specificity
This antibody reacts with beta2-microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with solublebeta2-microglobulin.
Form
Liquid
Conjugation
PE
Concentration
0.1 mg/mL
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using B2M monoclonal antibody, clone B2M-01 (Cat # MAB0899).
Lane 1 : RAJI (human Burkitt lymphoma cell line).
Lane 2 : EL4 (mouse lymphoblastic lymphoma cell line).
Lane 3 : U937 (human histiocytic lymphoma cell line).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
Flow Cytometry
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Gene Info — B2M
Entrez GeneID
567Gene Name
B2M
Gene Alias
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Gene Description
beta-2-microglobulin
Gene Ontology
HyperlinkGene Summary
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
Other Designations
beta chain of MHC class I molecules|beta-2-microglobin
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Interactome
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Pathway
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Disease
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