This antibody detects specifically target derived from human recombinant protein as well as Conditioned medium from LNCaP cells of at various concentrations. Cross reactivity with mouse and rat are not tested yet.
Protein G affinity chromatography
ELISA (1:1000000) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
Store at -20°C . After reconstitution with sterile PBS, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
The membrane blot was probed with B2M monoclonal antibody (Cat # MAB5428) (1 ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular recombinant B2M (12 KDa). Lane 1. Marker. Lane 2. HeLa cell lysate (induced). Lane 3. MD231. Lane 4. U251. Lane 5. Purified B2M protein.
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
beta chain of MHC class I molecules,beta-2-microglobin