CD40 monoclonal antibody, clone HI40a (FITC)
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More Files
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Flow Cytometry
Surface staining of human peripheral blood cells with CD40 monoclonal antibody, clone HI40a (FITC) (Cat # MAB5079). Cells in the lymphocyte gate were used for analysis.
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Specification
Product Description
Mouse monoclonal antibody raised against CD40a.
Immunogen
Human CD40a.
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Human
Specificity
This antibody recognizes CD40 (BP50), a 48 KDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells.
Form
Liquid
Conjugation
FITC
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Surface staining of human peripheral blood cells with CD40 monoclonal antibody, clone HI40a (FITC) (Cat # MAB5079). Cells in the lymphocyte gate were used for analysis. -
Gene Info — CD40
Entrez GeneID
958Gene Name
CD40
Gene Alias
Bp50, CDW40, MGC9013, TNFRSF5, p50
Gene Description
CD40 molecule, TNF receptor superfamily member 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
B cell surface antigen CD40|B cell-associated molecule|CD40 antigen|CD40 antigen (TNF receptor superfamily member 5)|CD40 type II isoform|CD40L receptor|OTTHUMP00000031699|OTTHUMP00000031700|nerve growth factor receptor-related B-lymphocyte activation mol
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Interactome
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Pathway
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Disease
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Publication Reference
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Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells.
Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB.
Toxicology and Applied Pharmacology 2007 Dec; 228(2):135.
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CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways.
Pearson LL, Castle BE, Kehry MR.
International Immunology 2001 Mar; 13(3):273.
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CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions.
Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF.
The Journal of Experimental Medicine 1996 May; 183(5):2209.
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Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells.
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