Mouse monoclonal antibody raised against native CD4.
Native purified CD4 from rabbit thymocytes.
The monoclonal antibody is directed against the CD4-antigen (T4-antigen), which is expressed on human peripheral T lymphocytes and 80% of thymocytes. The monoclonal antibody reacts on a low level with human monocytes and macrophages. The monoclonal antibody does not react with B-cells, granulocytes and thrombocytes. 60Kd MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD4 cluster and present on 54% of peripheral blood T lymphocytes, 50% of thymocytes and some malignant cells of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD4 antigen although cytoplasmic expression has been observed in monocytes/macrophages. The CD4 positive T lymphocyte subpopulation has been characterised functionally as comprising helper cells active in amplification of immune responses.
Flow Cytometry (20 ul/106 cells) The optimal working dilution should be determined by the end user.
In buffer containing 1% BSA, pH 7.2 (0.09% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.