5-mC monoclonal antibody, clone b
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More Files
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Immunofluorescence
Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).
A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.
B. Enlarged picture corresponding to a region from the left panel (as indicated in A).
C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
Immunoprecipitation
MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.
1 ug of antibody was used per IP experiment.
The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).
One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).
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Specification
Product Description
Mouse monoclonal antibody raised against 5-methyl Cytosine.
Immunogen
Ovalbumine conjugated with 5-methyl Cytosine.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (0.1-0.3 ug/mL)
Dot Blot (1-2 ug/mL)
Immunofluorescence (1:100)
Flow Cytometry (1-2 ug/mL)
Methylated DNA Immunoprecipitation (1-5 ug/IP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS
Storage Instruction
Aliquot and store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).
A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.
B. Enlarged picture corresponding to a region from the left panel (as indicated in A).
C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).Immunoprecipitation
MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.
1 ug of antibody was used per IP experiment.
The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).
One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).Enzyme-linked Immunoabsorbent Assay
Dot Blot
Methylated DNA Immunoprecipitation
Flow Cytometry
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